About this Dataset

Data From: Attraction, Mobility, And Preference By Lasioderma Serricorne (F.) (Coleoptera: Ptinidae) To Microbially-mediated Volatile Emissions By Two Species Of Fungi In Stored Grain

Our goals were to 1) isolate, and culture two fungal morphotypes, 2) characterize the volatile emissions from grain inoculated by each fungal morphotype (Aspergillus flavus or Fusarium spp.) compared to uninoculated and sanitized grain, and 3) understand how MVOCs from each morphotype affects mobility, attraction, and preference by L. serricorne. Headspace collection revealed that the Fusarium - and A. flavus -inoculated grain produced significantly different volatiles compared to sanitized grain or the positive control. Changes in MVOC emissions affected close-range foraging during an Ethovision assay, with a greater frequency of entering and spending time in a small zone with kernels inoculated with A. flavus compared to other treatments. In the release-recapture assay, MVOCs were found to be attractive to L. serricorne at a longer distances in commercial pitfall traps. While there was no preference shown among semiochemical stimuli in a still-air, four-way olfactometer, it is possible that methodological limitations prevented robust interpretation from this assay. Overall, our study suggests that MVOCs are important for close- and long-range orientation of L.serricorne during foraging, and that MVOCs may have the potential for inclusion in behaviorally-based tactics for this species.

Resources in this dataset:

Resource Title: 4-way olfactometer assay.

File Name: Sierra_2021_olfactometer_Ag_Data_commons.csv

Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel

Resource Title: Release-recapture Assay .

File Name: sierra_release_recapture_exp_2021_fungal_volatiles_agdata_commons.csv

Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel

Resource Title: Ethovision Movement Assay .

File Name: ethovision_sierra_2021_microbial_volatiles_agdatacommons.csv

Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel

Resource Title: Headspace volatile collection assay.

File Name: headspace_compounds_sierra_2021_fungal_volatiles_final_agdatacommons.csv

Resource Software Recommended: Microsoft Excel,url: https://www.microsoft.com/en-us/microsoft-365/excel

Resource Title: Sequencing Data.

File Name: sequencing_data.zip

Resource Title: File list.

File Name: File_list_L_serricone_attraction_data.txt
Organization: Department of Agriculture
Last updated: 2024-03-30T11:10:10.656999
Tags: ars, behavioral-ecology, coleoptera, data-gov, entomology, insects, ipm, microbes, mobility, mvocs, np304, olfaction, stored-products, trapping, volatile-responses

Tables

Ethovision Sierra 2021 Microbial Volatiles Agdatacommons

@usgov.usda_gov_data_from_attraction_mobility_and_preference__84c2d0bc.ethovision_sierra_2021_microbial_volatiles_agdatacommons

25.83 kB
79 rows
23 columns

CREATE TABLE ethovision_sierra_2021_microbial_volatiles_agdatacommons (
  "trial_no" VARCHAR  -- Trial No.,
  "arena_no" VARCHAR  -- Arena No.,
  "rep" BIGINT,
  "species" VARCHAR,
  "treatment" VARCHAR,
  "location_of_treatment" VARCHAR,
  "location_of_treatment_1" VARCHAR,
  "distance_moved_cm" DOUBLE  -- Distance Moved (cm),
  "velocity_cm_s" DOUBLE  -- Velocity (cm/s),
  "frequency_of_entering_kernel_zone_2" BIGINT,
  "cumulative_duration_in_kernel_zone_2_s" DOUBLE  -- Cumulative Duration In Kernel Zone 2 (s),
  "latency_to_first_entrance_of_kernel_zone_2_s" VARCHAR  -- Latency To First Entrance Of Kernel Zone 2 (s),
  "frequency_of_entering_kernel_zone_1" BIGINT,
  "cumulative_duration_in_kernel_zone_1_s" DOUBLE  -- Cumulative Duration In Kernel Zone 1 (s),
  "latency_to_first_entrance_of_kernel_zone_1_s" VARCHAR  -- Latency To First Entrance Of Kernel Zone 1 (s),
  "frequency_of_entering_zone_2" BIGINT,
  "cumulative_duration_in_zone_2_s" DOUBLE  -- Cumulative Duration In Zone 2 (s),
  "latency_to_first_entrance_of_zone_2_s" VARCHAR  -- Latency To First Entrance Of Zone 2 (s),
  "frequency_of_entering_zone_1" BIGINT,
  "cumulative_duration_in_zone_1_s" DOUBLE  -- Cumulative Duration In Zone 1 (s),
  "latency_to_first_entrance_of_zone_1_s" VARCHAR  -- Latency To First Entrance Of Zone 1 (s),
  "movement" DOUBLE,
  "turn_angle" VARCHAR
);

Headspace Compounds Sierra 2021 Fungal Volatiles Final Agdatacommons

@usgov.usda_gov_data_from_attraction_mobility_and_preference__84c2d0bc.headspace_compounds_sierra_2021_fungal_volatiles_final_21f92a27

50.99 kB
48 rows
61 columns

CREATE TABLE headspace_compounds_sierra_2021_fungal_volatiles_final_21f92a27 (
  "compound" VARCHAR,
  "ret_time" DOUBLE,
  "af_grain_rep_1" DOUBLE  -- AF-Grain: Rep 1,
  "af_grain_rep_2" DOUBLE  -- AF-Grain: Rep 2,
  "af_grain_rep_3" DOUBLE  -- AF-Grain: Rep 3,
  "af_grain_rep_4" DOUBLE  -- AF-Grain: Rep 4,
  "af_grain_rep_5" DOUBLE  -- AF-Grain: Rep 5,
  "af_grain_rep_6" DOUBLE  -- AF-Grain: Rep 6,
  "af_grain_rep_7" DOUBLE  -- AF-Grain: Rep 7,
  "fs_grain_rep_1" DOUBLE  -- FS-Grain: Rep 1,
  "fs_grain_rep_2" DOUBLE  -- FS-Grain: Rep 2,
  "fs_grain_rep_3" DOUBLE  -- FS-Grain: Rep 3,
  "fs_grain_rep_4" DOUBLE  -- FS-Grain: Rep 4,
  "fs_grain_rep_5" DOUBLE  -- FS-Grain: Rep 5,
  "fs_grain_rep_6" DOUBLE  -- FS-Grain: Rep 6,
  "p_ctrl_rep_1" DOUBLE  -- P. Ctrl: Rep 1,
  "p_ctrl_rep_2" DOUBLE  -- P. Ctrl: Rep 2,
  "p_ctrl_rep_3" DOUBLE  -- P. Ctrl: Rep 3,
  "p_ctrl_rep_4" DOUBLE  -- P. Ctrl: Rep 4,
  "p_ctrl_rep_5" DOUBLE  -- P. Ctrl: Rep 5,
  "p_ctrl_rep_6" DOUBLE  -- P. Ctrl: Rep 6,
  "uv_grain_rep_1" DOUBLE  -- UV-Grain: Rep 1,
  "uv_grain_rep_2" DOUBLE  -- UV-Grain: Rep 2,
  "uv_grain_rep_3" DOUBLE  -- UV-Grain: Rep 3,
  "uv_grain_rep_4" DOUBLE  -- UV-Grain: Rep 4,
  "uv_grain_rep_6" DOUBLE  -- UV-Grain: Rep 6,
  "uv_grain_rep_7" DOUBLE  -- UV-Grain: Rep 7,
  "neg_ctrl_rep_1" DOUBLE  -- Neg. Ctrl: Rep 1,
  "neg_ctrl_rep_2" DOUBLE  -- Neg. Ctrl: Rep 2,
  "neg_ctrl_rep_3" DOUBLE  -- Neg. Ctrl: Rep 3,
  "neg_ctrl_rep_4" DOUBLE  -- Neg. Ctrl: Rep 4,
  "neg_ctrl_rep_5" DOUBLE  -- Neg. Ctrl: Rep 5,
  "neg_ctrl_rep_6" DOUBLE  -- Neg. Ctrl: Rep 6,
  "unnamed_33" VARCHAR  -- Unnamed: 33,
  "unnamed_34" VARCHAR  -- Unnamed: 34,
  "unnamed_35" VARCHAR  -- Unnamed: 35,
  "unnamed_36" VARCHAR  -- Unnamed: 36,
  "unnamed_37" VARCHAR  -- Unnamed: 37,
  "p_ctrl" VARCHAR  -- P. Ctrl,
  "unnamed_39" VARCHAR  -- Unnamed: 39,
  "unnamed_40" VARCHAR  -- Unnamed: 40,
  "unnamed_41" VARCHAR  -- Unnamed: 41,
  "unnamed_42" VARCHAR  -- Unnamed: 42,
  "unnamed_43" VARCHAR  -- Unnamed: 43,
  "uv_grain" VARCHAR,
  "unnamed_45" VARCHAR  -- Unnamed: 45,
  "unnamed_46" VARCHAR  -- Unnamed: 46,
  "unnamed_47" VARCHAR  -- Unnamed: 47,
  "unnamed_48" VARCHAR  -- Unnamed: 48,
  "unnamed_49" VARCHAR  -- Unnamed: 49,
  "a_flavus_grain" VARCHAR  -- A. Flavus-Grain,
  "unnamed_51" VARCHAR  -- Unnamed: 51,
  "unnamed_52" VARCHAR  -- Unnamed: 52,
  "unnamed_53" VARCHAR  -- Unnamed: 53,
  "unnamed_54" VARCHAR  -- Unnamed: 54,
  "unnamed_55" VARCHAR  -- Unnamed: 55,
  "fusarium_spp_grain" VARCHAR,
  "unnamed_57" VARCHAR  -- Unnamed: 57,
  "unnamed_58" VARCHAR  -- Unnamed: 58,
  "unnamed_59" VARCHAR  -- Unnamed: 59,
  "unnamed_60" VARCHAR  -- Unnamed: 60
);

Sierra Release Recapture Exp 2021 Fungal Volatiles Agdata Commons

@usgov.usda_gov_data_from_attraction_mobility_and_preference__84c2d0bc.sierra_release_recapture_exp_2021_fungal_volatiles_agd_a08b2a5e

5.32 kB
40 rows
6 columns

CREATE TABLE sierra_release_recapture_exp_2021_fungal_volatiles_agd_a08b2a5e (
  "date" TIMESTAMP,
  "rep" BIGINT,
  "treatment" VARCHAR,
  "n__recaptured" BIGINT  -- # Recaptured,
  "n__released" BIGINT  -- # Released,
  "percent_recaptured" BIGINT
);