About this Dataset

Dataset From HDX-MS Studies Of IgG1 Glycoforms And Their Interactions With The Fc(gamma)RIa (CD64) Receptor

This database gives hydrogen-deuterium exchange mass spectrometry (HDX-MS) data from measurements of three purified IgG1 glycoform samples, predominantly G0F, G2F, and SAF, in isolation and in complexation with the high-affinity receptor, Fc(gamma)RIa (CD64). The IgG1 antibody used in this study, aIL8hFc, is a murine-human chimeric IgG1, which inhibits IL-8 binding to human neutrophils. The working datasets comprise 17,750 deuterium uptake measurements in peptides from the receptor and the light and heavy chains of aIL8hFc obtained during 7 experiments, each comprising three runs of six exchange times. The data illuminate on the hydrogen exchange dynamics in isolated receptor, isolated IgG1 glycoforms, and receptor-IgG1 complexes.
Organization: National Institute of Standards and Technology
Last updated: 2021-06-04T22:24:28.236156
Tags: antibody-receptor-interaction, chromatography, glycosylation, hdx-ms, hydrogen-deuterium-exchange, mass-spectrometry, monoclonal-antibody, peptide, precision, protein, proteolysis, proteomics, receptor

Tables

HDX-MS Data Of Peptides From Isolated (apo-)Fc?R1a.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_data_of_peptides_from_isolated_apo_fc_r1a

15.19 kB
791 rows
9 columns

CREATE TABLE hdx_ms_data_of_peptides_from_isolated_apo_fc_r1a (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From (holo-)Fc(gamma)R1a In Complexation With AIL8hFc SAF Glycoform.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_holo_fc_gamma_r1a_in_complexa_739e805b

17.16 kB
899 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_holo_fc_gamma_r1a_in_complexa_739e805b (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From (holo-)Fc(gamma)R1a In Complexation With AIL8hFc G2F Glycoform.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_holo_fc_gamma_r1a_in_complexa_83a56e8f

16.83 kB
881 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_holo_fc_gamma_r1a_in_complexa_83a56e8f (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From (holo-)Fc(gamma)R1a In Complexation With AIL8hFc G0F Glycoform.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_holo_fc_gamma_r1a_in_complexa_f818e8c9

17.78 kB
933 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_holo_fc_gamma_r1a_in_complexa_f818e8c9 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From The Heavy Chain Of Holo-aIL8hFc SAF Glycoform In Complexation With Fc(gamma)R1a.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_the_heavy_chain_of_holo_ail8h_d35ad697

22.51 kB
1,548 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_the_heavy_chain_of_holo_ail8h_d35ad697 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From The Heavy Chain Of Holo-aIL8hFc G0F Glycoform In Complexation With Fc(gamma)R1a.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_the_heavy_chain_of_holo_ail8h_f3fc36e1

21.98 kB
1,494 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_the_heavy_chain_of_holo_ail8h_f3fc36e1 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From The Heavy Chain Of Holo-aIL8hFc G2F Glycoform In Complexation With Fc(gamma)R1a.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_the_heavy_chain_of_holo_ail8h_f60e62a1

22.52 kB
1,566 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_the_heavy_chain_of_holo_ail8h_f60e62a1 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From The Light Chain Of Holo-aIL8hFc SAF Glycoform In Complexation With Fc(gamma)R1a.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_the_light_chain_of_holo_ail8h_31ea6e1b

17.25 kB
986 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_the_light_chain_of_holo_ail8h_31ea6e1b (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From The Light Chain Of Holo-aIL8hFc G2F Glycoform In Complexation With Fc(gamma)R1a.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_the_light_chain_of_holo_ail8h_96778445

17.09 kB
980 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_the_light_chain_of_holo_ail8h_96778445 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data For Peptides From The Light Chain Of Holo-aIL8hFc G0F Glycoform In Complexation With Fc(gamma)R1a .

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_for_peptides_from_the_light_chain_of_holo_ail8h_ef82e879

16.62 kB
916 rows
9 columns

CREATE TABLE hdx_ms_for_peptides_from_the_light_chain_of_holo_ail8h_ef82e879 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data Of Peptides From The Heavy Chain Of Isolated (apo-)G0F IgG1 AIL8hFc.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_of_peptides_from_the_heavy_chain_of_isolated_ap_644b8e9c

20.69 kB
1,367 rows
9 columns

CREATE TABLE hdx_ms_of_peptides_from_the_heavy_chain_of_isolated_ap_644b8e9c (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data Of Peptides From The Heavy Chain Of Isolated (apo-)G2F IgG1 AIL8hFc.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_of_peptides_from_the_heavy_chain_of_isolated_ap_c775fc4d

21.73 kB
1,384 rows
9 columns

CREATE TABLE hdx_ms_of_peptides_from_the_heavy_chain_of_isolated_ap_c775fc4d (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data Of Peptides From The Heavy Chain Of Isolated (apo-)SAF IgG1 AIL8hFc.

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_of_peptides_from_the_heavy_chain_of_isolated_ap_da8f3ab7

21.9 kB
1,494 rows
9 columns

CREATE TABLE hdx_ms_of_peptides_from_the_heavy_chain_of_isolated_ap_da8f3ab7 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data Of Peptides From The Light Chain Of Isolated (apo-)SAF IgG1 AIL8hFc

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_of_peptides_from_the_light_chain_of_isolated_ap_282e8eab

16.82 kB
933 rows
9 columns

CREATE TABLE hdx_ms_of_peptides_from_the_light_chain_of_isolated_ap_282e8eab (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data Of Peptides From The Light Chain Of Isolated (apo-)G0F IgG1 AIL8hFc

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_of_peptides_from_the_light_chain_of_isolated_ap_916f5a5b

14.77 kB
660 rows
9 columns

CREATE TABLE hdx_ms_of_peptides_from_the_light_chain_of_isolated_ap_916f5a5b (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);

HDX-MS Data Of Peptides From The Light Chain Of Isolated (apo-)G2F IgG1 AIL8hFc

@usgov.national_institute_of_standard_dataset_from_hdx_ms_stu_b7bd9d17.hdx_ms_of_peptides_from_the_light_chain_of_isolated_ap_c78e6d90

16.45 kB
916 rows
9 columns

CREATE TABLE hdx_ms_of_peptides_from_the_light_chain_of_isolated_ap_c78e6d90 (
  "start" BIGINT,
  "end" BIGINT,
  "sequence" VARCHAR,
  "charge" BIGINT,
  "mono_mass_da" DOUBLE  -- Mono-Mass(Da),
  "rt_window_min" VARCHAR  -- RT Window (min),
  "time_s" BIGINT  -- Time(s),
  "mass_uptake_da" DOUBLE  -- Mass Uptake(Da),
  "unnamed_8" VARCHAR  -- Unnamed: 8
);