About this Dataset

Data From: Venereal Transmission Of Vesicular Stomatitis Virus By Culicoides Sonorensis Midges

Culicoides sonorensis biting midges are well-known agricultural pests and transmission vectors of arboviruses such as vesicular stomatitis virus (VSV). The epidemiology of VSV is complex and encompasses a broad range of vertebrate hosts, multiple routes of transmission, and diverse vector species. In temperate regions, viruses can overwinter in the absence of infected animals through unknown mechanisms, to reoccur the next year. Non-conventional routes for VSV vector transmission may help explain viral maintenance in midge populations during inter-epidemic periods and times of adverse conditions for bite transmission. In this study, we examined whether VSV could be transmitted venereally between male and female midges. Our results showed that VSV-infected females could venereally transmit virus to uninfected naïve males at a rate as high as 76.3% (RT-qPCR), 31.6% (virus isolation) during the third gonotrophic cycle. Additionally, VSV-infected males could venereally transmit virus to uninfected naïve females at a rate as high as 76.6% (RT-qPCR), 49.2% (virus isolation). Immunofluorescent staining of micro-dissected reproductive organs, immunochemical staining of midge histological sections, examination of internal reproductive organ morphology, and observations of mating behaviors were used to determine relevant anatomical sites for virus location and to hypothesize the potential mechanism for VSV transmission in C. sonorensis midges through copulation.
Organization: Department of Agriculture
Last updated: 2024-03-30T11:27:58.754399
Tags: culicoides-midges, mating-behavior, non-conventional-transmission, reproductive-anatomy, venereal-transmission, vesicular-stomatitis-virus

Tables

RT QPCR Data Fig2b

@usgov.usda_gov_data_from_venereal_transmission_of_vesicular__96f91835.rt_qpcr_data_fig2b

4.29 kB
35 rows
3 columns

CREATE TABLE rt_qpcr_data_fig2b (
  "cycle_threshold_values_of_reverse_transcription_quanti_daae8c4a" VARCHAR  -- Cycle Threshold Values Of Reverse-transcription - Quantitative Polymerase Chain Reaction (RT-qPCR) Amplification For Vesicular Stomatitis Virus (VSV) In Individual Female C. Sonorensis Midges Following Oral Infection At The End Of One, Two, Or Three Gonotrophic Cycles (1 GC, 2 GC, 3 GC),
  "unnamed_1" VARCHAR  -- Unnamed: 1,
  "unnamed_2" VARCHAR  -- Unnamed: 2
);

RT QPCR Data Fig3

@usgov.usda_gov_data_from_venereal_transmission_of_vesicular__96f91835.rt_qpcr_data_fig3

3.69 kB
61 rows
2 columns

CREATE TABLE rt_qpcr_data_fig3 (
  "cycle_threshold_values_of_reverse_transcription_quanti_0b90b7b6" VARCHAR  -- Cycle Threshold Values Of Reverse-transcription - Quantitative Polymerase Chain Reaction (RT-qPCR) Amplification For Vesicular Stomatitis Virus (VSV) In Individual Heads And Bodies Of Female C. Sonorensis Midges After Mating With Males That Had Been Infected With VSV By Microinjection,
  "unnamed_1" VARCHAR  -- Unnamed: 1
);

RT QPCR Data Fig4

@usgov.usda_gov_data_from_venereal_transmission_of_vesicular__96f91835.rt_qpcr_data_fig4

2.57 kB
10 rows
1 column

CREATE TABLE rt_qpcr_data_fig4 (
  "cycle_threshold_values_of_reverse_transcription_quanti_68a97a4f" VARCHAR  -- Cycle Threshold Values Of Reverse-transcription - Quantitative Polymerase Chain Reaction (RT-qPCR) Amplification For Vesicular Stomatitis Virus (VSV) In Individual Female C. Sonorensis Midges After Mating With Males That Had Been Venereally Infected With VSV
);