About this Dataset

Data From: Toll-like Receptor Ligands Enhance Vaccine Efficacy Against Virulent Newcastle Disease Virus Challenge In Chickens

To enhance the efficacy of the current Newcastle disease vaccine, we have tested two potential adjuvants (Imiquimod and ODN-1826) in chickens. Birds were treated with PBS, Imiquimod or ODN-1826 (50 µg/bird) or vaccinated intranasally with live LaSota strain with or without Imiquimod or ODN-1826 (50 µg/bird). Two weeks after vaccination, birds were challenged with virulent New-castle disease virus (chicken/CA/212676/2002). The experimental setting was a laboratory with animal care as approved by the institutional animal care and use committee as appropriate for the species and age of bird. Data are the serum antibody titers to the vaccine or challenge virus as determined by hemagglutination inhibition assay. Virus shedding titers by the oral and cloacal route for individual birds exposed to virulent Newcastle disease virus were determined by quantitative real-time RT-PCR. Expression of antiviral genes from tissues collected at 1 and 3 days after treating 2 week old SPF chickens with adjuvant and/or vaccines were determined by quantitative PCR.

Resources in this dataset:

Hemagglutination inhibition (HI) antibody titers 14 days after vaccinating 2-week-old SPF chickens with LaSota vaccine with or without adjuvant (ODN-1826 or Imiquimod) File Name: Lee et al., Serology Data I from Adjuvanted live NDV vaccine study.csv
Hemagglutination inhibition antibody titers 14 days after infecting 4 week old SPF chickens with virulent Newcastle disease virus (California/212676/2002) File Name: Lee et al., Serology Data II from Adjuvanted live NDV vaccine study.csv
Cycle threshold (Ct) value of viral RNA from swabs collected at 2 and 4 days after infecting 4-week-old SPF chickens with virulent Newcastle disease virus (California/212676/2002) File Name: Lee et al.,Virus Shedding Data from Adjuvanted live NDV vaccine study.csv
Cycle threshold (Ct) value of target specific mRNA collected at 1 and 3 days after treating 2-week-old SPF chickens with adjuvant and/or vaccines File Name: Lee et al.,Transcription Data from Adjuvanted live NDV vaccine study.csv
Organization: Department of Agriculture
Last updated: 2024-03-30T11:26:40.613728
Tags: antibody-response, antiviral-response, ars, data-gov, newcastle-disease, np103, vaccine

Tables

Lee Et Al., Serology Data II From Adjuvanted Live NDV Vaccine Study

@usgov.usda_gov_data_from_toll_like_receptor_ligands_enhance__55beb61e.lee_et_al_serology_ii_from_adjuvanted_live_ndv_vaccine_study

4.92 KB
63 rows
5 columns


CREATE TABLE lee_et_al_serology_ii_from_adjuvanted_live_ndv_vaccine_study (
  "serology_ii_from_lee_et_al_toll_like_receptor_ligands__63b0b2c2" VARCHAR,
  "unnamed_1" VARCHAR,
  "unnamed_2" VARCHAR,
  "unnamed_3" VARCHAR,
  "unnamed_4" VARCHAR
);

Lee Et Al.,Transcription Data From Adjuvanted Live NDV Vaccine Study

@usgov.usda_gov_data_from_toll_like_receptor_ligands_enhance__55beb61e.lee_et_al_transcription_from_adjuvanted_live_ndv_vaccine_study

12.24 KB
78 rows
11 columns


CREATE TABLE lee_et_al_transcription_from_adjuvanted_live_ndv_vaccine_study (
  "transcription_from_lee_et_al_toll_like_receptor_ligand_74db3472" VARCHAR,
  "unnamed_1" VARCHAR,
  "unnamed_2" VARCHAR,
  "unnamed_3" VARCHAR,
  "unnamed_4" VARCHAR,
  "unnamed_5" VARCHAR,
  "unnamed_6" VARCHAR,
  "unnamed_7" VARCHAR,
  "unnamed_8" VARCHAR,
  "unnamed_9" VARCHAR,
  "unnamed_10" VARCHAR
);

Lee Et Al.,Virus Shedding Data From Adjuvanted Live NDV Vaccine Study

@usgov.usda_gov_data_from_toll_like_receptor_ligands_enhance__55beb61e.lee_et_al_virus_shedding_from_adjuvanted_live_ndv_vaccine_study

6.5 KB
69 rows
6 columns


CREATE TABLE lee_et_al_virus_shedding_from_adjuvanted_live_ndv_vaccine_study (
  "virus_shedding_from_lee_et_al_toll_like_receptor_ligan_de41e67e" VARCHAR,
  "unnamed_1" VARCHAR,
  "unnamed_2" VARCHAR,
  "unnamed_3" VARCHAR,
  "unnamed_4" VARCHAR,
  "unnamed_5" VARCHAR
);