Data From: An In Vitro Analysis Of How Lactose Modifies The Gut Microbiota Structure And Function Of Adults In A Donor-independent Manner

The impact of lactose on the gut microbiota of healthy adults was examined, using a short-term, in vitro strategy where fecal samples harvested from 18 donors were cultured anaerobically with and without lactose. Donors represented 3 adult age groups. Data collected include: amplicon sequencing of the V1-V2 regions of the 16S rRNA gene (available in the NCBI Sequence Read Archive associated with BioProject PRJNA883645: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA883645), RT-qPCR of Bifidobacterium 16S rRNA genes, and short-chain fatty acid concentrations.

Resources in this dataset:

• Resource Title: lactose sample attributes File Name: lactose_sample_metadata.csv

• Resource Title: lactose SCFA, pH, and gas production data File Name: lactose_SCFA_data.csv

• Resource Title: Total bacterial and Bifidobacterium genus qPCR File Name: lactose_qPCR_all_bacteria_and_bifido.csv
  Organization: Department of Agriculture
  Organization URL: https://catalog.data.gov/organization/usda
  Last updated: 2025-11-21
  Tags: 16S rRNA sequence data, ARS, NP306, data.gov, lactose, short chain fatty acids